prikaz prve stranice dokumenta Estimating quality of archive urban stream macroinvertebrate samples for genomic, transcriptomic and proteomic assessment
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Scientific paper - Original scientific paper
Estimating quality of archive urban stream macroinvertebrate samples for genomic, transcriptomic and proteomic assessment
Ecological Indicators, 125 (2021); 107509. https://doi.org/10.1016/j.ecolind.2021.107509
Begić, Valerija; Sertić Perić, Mirela; Hančić, Suzana; Štargl, Mihaela; Svoboda, Matea; Korać, Petra; Radanović, Ines

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Begić, V., Sertić Perić, M., Hančić, S., Štargl, M., Svoboda, M., Korać, P. & Radanović, I. (2021). Estimating quality of archive urban stream macroinvertebrate samples for genomic, transcriptomic and proteomic assessment. Ecological Indicators, 125., 107509. doi: 10.1016/j.ecolind.2021.107509

Begić, Valerija, et al. "Estimating quality of archive urban stream macroinvertebrate samples for genomic, transcriptomic and proteomic assessment." Ecological Indicators, vol. 125, 2021, p. 107509. https://doi.org/10.1016/j.ecolind.2021.107509

Begić, Valerija, Mirela Sertić Perić, Suzana Hančić, Mihaela Štargl, Matea Svoboda, Petra Korać and Ines Radanović. "Estimating quality of archive urban stream macroinvertebrate samples for genomic, transcriptomic and proteomic assessment." Ecological Indicators 125 (2021): 107509. https://doi.org/10.1016/j.ecolind.2021.107509

Begić, V., et al. (2021) 'Estimating quality of archive urban stream macroinvertebrate samples for genomic, transcriptomic and proteomic assessment', Ecological Indicators, 125, p. 107509. doi: 10.1016/j.ecolind.2021.107509

Begić V, Sertić Perić M, Hančić S, Štargl M, Svoboda M, Korać P, and sur.. Estimating quality of archive urban stream macroinvertebrate samples for genomic, transcriptomic and proteomic assessment. Ecological Indicators [Internet]. 2021 June [cited 2024 December 19];125:107509. doi: 10.1016/j.ecolind.2021.107509

V. Begić, et al., "Estimating quality of archive urban stream macroinvertebrate samples for genomic, transcriptomic and proteomic assessment", Ecological Indicators, vol. 125, p. 107509, June 2021. [Online]. Available at: https://urn.nsk.hr/urn:nbn:hr:264:283617. [Accessed: 19 December 2024]

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Metadata
Title (english)Estimating quality of archive urban stream macroinvertebrate samples for genomic, transcriptomic and proteomic assessment
AuthorValerija Begić
AuthorMirela Sertić Perić
AuthorSuzana Hančić
AuthorMihaela Štargl
AuthorMatea Svoboda
AuthorPetra Korać
AuthorInes Radanović
Author's institution(Merkur University Hospital)
Scientific / art field,
discipline and subdiscipline		NATURAL SCIENCES
Biology
Abstract (english)
The existence of archival collections of macroinvertebrates opens the possibility of using these organisms as biological indicators and model organisms for developing indices of aquatic ecosystem health and stress, which provide a powerful basis for sustainable environmental monitoring and management of water resources. The environmental assessment of urbanization influence on urban stream biota over time can include molecular (proteomic, transcriptomic and genomic) analyses of aquatic insects isolated from archive samples. These analyses could include confirmation of detected species by barcoding, determination of the changes in the expression of specific genes and detection of changes in signaling pathways in cells of the studied organisms. Since the preservation of the organisms’ macromolecules (DNA, RNA and proteins) is a prerequisite for the implementation of molecular methods, the aim of this study was to assess the quality of macromolecule preservation within archival samples of aquatic macroinvertebrates collected in urban streams during last 28 years. Results indicated that the optimal way for archive storage is 3.6% formaldehyde solution if they have a purpose of morphological assessment or protein analyses, and in absolute ethanol for nucleic acids evaluation. Furthermore, it is of great importance that samples are treated with fixative in situ and processed in a laboratory as soon as possible.
Keywords (english)
Languageenglish
Publication typeScientific paper - Original scientific paper
Publication statusPublished
Peer reviewPeer review - international
Publication versionPublished version
Journal titleEcological Indicators
Numberingvol. 125, p. 107509
p-ISSN1470-160X
DOIhttps://doi.org/10.1016/j.ecolind.2021.107509
URN:NBNurn:nbn:hr:264:283617
Publication2021-06
Document URLhttps://linkinghub.elsevier.com/retrieve/pii/S1470160X21001746
Type of resourceText
Access conditionsOpen access
Terms of useLicence Attribution 4.0 International (CC BY 4.0) icon
Created on2024-12-04 13:45:53