prikaz prve stranice dokumenta Estimating quality of archive urban stream macroinvertebrate samples for genomic, transcriptomic and proteomic assessment
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Estimating quality of archive urban stream macroinvertebrate samples for genomic, transcriptomic and proteomic assessment
Ecological Indicators, 125 (2021); 107509. https://doi.org/10.1016/j.ecolind.2021.107509
Begić, Valerija; Sertić Perić, Mirela; Hančić, Suzana; Štargl, Mihaela; Svoboda, Matea; Korać, Petra; Radanović, Ines

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Begić, V., Sertić Perić, M., Hančić, S., Štargl, M., Svoboda, M., Korać, P. i Radanović, I. (2021). Estimating quality of archive urban stream macroinvertebrate samples for genomic, transcriptomic and proteomic assessment. Ecological Indicators, 125., 107509. doi: 10.1016/j.ecolind.2021.107509

Begić, Valerija, et al. "Estimating quality of archive urban stream macroinvertebrate samples for genomic, transcriptomic and proteomic assessment." Ecological Indicators, vol. 125, 2021, str. 107509. https://doi.org/10.1016/j.ecolind.2021.107509

Begić, Valerija, Mirela Sertić Perić, Suzana Hančić, Mihaela Štargl, Matea Svoboda, Petra Korać i Ines Radanović. "Estimating quality of archive urban stream macroinvertebrate samples for genomic, transcriptomic and proteomic assessment." Ecological Indicators 125 (2021): 107509. https://doi.org/10.1016/j.ecolind.2021.107509

Begić, V., et al. (2021) 'Estimating quality of archive urban stream macroinvertebrate samples for genomic, transcriptomic and proteomic assessment', Ecological Indicators, 125, str. 107509. doi: 10.1016/j.ecolind.2021.107509

Begić V, Sertić Perić M, Hančić S, Štargl M, Svoboda M, Korać P, i sur.. Estimating quality of archive urban stream macroinvertebrate samples for genomic, transcriptomic and proteomic assessment. Ecological Indicators [Internet]. 06.2021. [pristupljeno 27.01.2025.];125:107509. doi: 10.1016/j.ecolind.2021.107509

V. Begić, et al., "Estimating quality of archive urban stream macroinvertebrate samples for genomic, transcriptomic and proteomic assessment", Ecological Indicators, vol. 125, str. 107509, Lipanj 2021. [Online]. Dostupno na: https://urn.nsk.hr/urn:nbn:hr:264:283617. [Citirano: 27.01.2025.]

Za citiranje koristite ovu mrežnu adresu: https://urn.nsk.hr/urn:nbn:hr:264:283617
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Podaci o radu
Naslov (engleski)Estimating quality of archive urban stream macroinvertebrate samples for genomic, transcriptomic and proteomic assessment
AutorValerija Begić
AutorMirela Sertić Perić
AutorSuzana Hančić
AutorMihaela Štargl
AutorMatea Svoboda
AutorPetra Korać
AutorInes Radanović
Autorova ustanova(Klinička bolnica Merkur)
Znanstveno / umjetničko
područje, polje i grana		PRIRODNE ZNANOSTI
Biologija
Sažetak (engleski)
The existence of archival collections of macroinvertebrates opens the possibility of using these organisms as biological indicators and model organisms for developing indices of aquatic ecosystem health and stress, which provide a powerful basis for sustainable environmental monitoring and management of water resources. The environmental assessment of urbanization influence on urban stream biota over time can include molecular (proteomic, transcriptomic and genomic) analyses of aquatic insects isolated from archive samples. These analyses could include confirmation of detected species by barcoding, determination of the changes in the expression of specific genes and detection of changes in signaling pathways in cells of the studied organisms. Since the preservation of the organisms’ macromolecules (DNA, RNA and proteins) is a prerequisite for the implementation of molecular methods, the aim of this study was to assess the quality of macromolecule preservation within archival samples of aquatic macroinvertebrates collected in urban streams during last 28 years. Results indicated that the optimal way for archive storage is 3.6% formaldehyde solution if they have a purpose of morphological assessment or protein analyses, and in absolute ethanol for nucleic acids evaluation. Furthermore, it is of great importance that samples are treated with fixative in situ and processed in a laboratory as soon as possible.
Ključne riječi (engleski)
Jezikengleski
Vrsta publikacijeZnanstveni rad - Izvorni znanstveni rad
Status objaveObjavljen
Vrsta recenzijeRecenziran - međunarodna recenzija
Verzija publikacijeObjavljena verzija rada (izdavačev PDF)
Naslov časopisaEcological Indicators
Brojčani podacivol. 125, str. 107509
p-ISSN1470-160X
DOIhttps://doi.org/10.1016/j.ecolind.2021.107509
URN:NBNurn:nbn:hr:264:283617
Datum objave publikacije2021-06
URL dokumentahttps://linkinghub.elsevier.com/retrieve/pii/S1470160X21001746
Vrsta resursaTekst
Prava pristupaOtvoreni pristup
Uvjeti korištenjaIkona licence Imenovanje 4.0 međunarodna (CC BY 4.0)
Datum i vrijeme pohrane2024-12-04 13:45:53